A new ELISA and western blot technique based on recombinant TES antigen and/or larval antigen for the detection of toxocariasis in humans.
作者： Marie-Kristin Raulf , Daniela Jordan , Herbert Auer , Jens M. Warnecke , Bernd Lepenies
DOI: 10.1017/S0031182020002085
关键词:
摘要: Serological antibody detection by enzyme-linked immunosorbent assay (ELISA)- and immunoblot-based methods constitutes the best indicator of human Toxocara infection. Nevertheless, availability serological tests, particularly western blots (WB), evaluated for sensitivity specificity is limited. Therefore, an Anti-Toxocara-ELISA immunoglobulin g (IgG) prototype (Proto-ELISA) Anti-Toxocara-Westernblot (Proto-WB) were testing 541 sera pre-determined infection established in-house (IH-ELISA). To evaluate newly developed ELISA WB prototypes, results compared to IH-ELISA a commercial (Com-WB). Compared IH-ELISA, 93.1% (229/246) 94.6% (279/295) Proto-ELISA with Cohen's κ 0.88 obtained. The Proto-WB was 76.7% (240/313) 99.6% (227/228) 0.73 those Com-WB. A comparison revealed 91.5% (225/246) 0.86. Cross-reactivity observed some samples positive Ascaris Trichinella spp. in Proto-ELISA, Overall, prototypes showed high specificity, indicating reliability these tests.
elsevier.com
sci-hub.st 参考文章(47)
T. J. Murphy, E. G. D. Cohen, On the Sequences of Collisions Among Hard Spheres in Infinite Space Springer, Berlin, Heidelberg. pp. 29- 49 ,(2000) , 10.1007/978-3-662-04062-1_3
H. Smith, R. Noordin, Diagnostic limitations and future trends in the serodiagnosis of human toxocariasis. Toxocara: the enigmatic parasite. pp. 89- 112 ,(2005) , 10.1079/9781845930264.0089
P Jacquier, B Gottstein, Y Stingelin, J Eckert, Immunodiagnosis of toxocarosis in humans: evaluation of a new enzyme-linked immunosorbent assay kit. Journal of Clinical Microbiology. ,vol. 29, pp. 1831- 1835 ,(1991) , 10.1128/JCM.29.9.1831-1835.1991
Steven Van Den Broucke, Kirezi Kanobana, Katja Polman, Patrick Soentjens, Marc Vekemans, Caroline Theunissen, Erika Vlieghe, Marjan Van Esbroeck, Jan Jacobs, Erwin Van Den Enden, Jef Van Den Ende, Alfons Van Gompel, Jan Clerinx, Emmanuel Bottieau, Toxocariasis diagnosed in international travelers at the Institute of Tropical Medicine, Antwerp, Belgium, from 2000 to 2013. PLOS Neglected Tropical Diseases. ,vol. 9, pp. 1- 15 ,(2015) , 10.1371/JOURNAL.PNTD.0003559
G. Rubinsky-Elefant, C. E. Hirata, J. H. Yamamoto, M. U. Ferreira, Human toxocariasis: diagnosis, worldwide seroprevalences and clinical expression of the systemic and ocular forms Annals of Tropical Medicine and Parasitology. ,vol. 104, pp. 3- 23 ,(2010) , 10.1179/136485910X12607012373957
T. J. Murphy, E. G. D. Cohen, Maximum number of collisions among identical hard spheres Journal of Statistical Physics. ,vol. 71, pp. 1063- 1080 ,(1993) , 10.1007/BF01049961
Rosanna Qualizza, Cristoforo Incorvaia, Romualdo Grande, Eleni Makri, Luigi Allegra, Seroprevalence of IgG anti-Toxocara species antibodies in a population of patients with suspected allergy. International Journal of General Medicine. ,vol. 4, pp. 783- 787 ,(2011) , 10.2147/IJGM.S24324
Mohammad Zibaei, Farzaneh Firoozeh, Parviz Bahrami, Seyed Mahmoud Sadjjadi, Investigation of Anti-Toxocara Antibodies in Epileptic Patients and Comparison of Two Methods: ELISA and Western Blotting Epilepsy Research and Treatment. ,vol. 2013, pp. 156815- 156815 ,(2013) , 10.1155/2013/156815
Dorn Watthanakulpanich, Huw V. Smith, Glyn Hobbs, Anthony J. Whalley, David Billington, Application of Toxocara canis excretory–secretory antigens and IgG subclass antibodies (IgG1-4) in serodiagnostic assays of human toxocariasis Acta Tropica. ,vol. 106, pp. 90- 95 ,(2008) , 10.1016/J.ACTATROPICA.2008.01.008
S. Mohamad, N. C. Azmi, R. Noordin, Development and Evaluation of a Sensitive and Specific Assay for Diagnosis of Human Toxocariasis by Use of Three Recombinant Antigens (TES-26, TES-30USM, and TES-120) Journal of Clinical Microbiology. ,vol. 47, pp. 1712- 1717 ,(2009) , 10.1128/JCM.00001-09