The eukaryotic polypeptide chain releasing factor (eRF3/GSPT) carrying the translation termination signal to the 3'-Poly(A) tail of mRNA. Direct association of erf3/GSPT with polyadenylate-binding protein.
作者： Shin-ichi Hoshino , Mariko Imai , Tetsuo Kobayashi , Naoyuki Uchida , Toshiaki Katada
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摘要: The mammalian GTP-binding protein GSPT, whose carboxyl-terminal sequence is homologous to the eukaryotic elongation factor EF1α, binds polypeptide chain releasing eRF1 function as eRF3 in translation termination. amino-terminal domain of GSPT was, however, not required for binding. Search other GSPT-binding proteins yeast two-hybrid screening system resulted identification a cDNA encoding polyadenylate-binding (PABP), amino terminus associating with poly(A) tail mRNAs presumably their stabilization. interaction appeared be mediated through PABP and region GSPT. Interestingly, multimerization poly(A), which ascribed action its domain, was completely inhibited by These results indicate that GSPT/eRF3 may play important roles only termination synthesis but also regulation mRNA stability. Thus, present study first report showing carries signal 3′-poly(A) ubiquitously mRNAs.
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