Integrated capture and amplification of target nucleic acid for sequencing

摘要: The invention provides efficient methods of preparing a target nucleic acid in form suitable for sequencing. are particularly amenable high quality acids massively parallel involve capturing from sample and PCR amplification the acid. is captured by binding to capture probe, which turn binds an immobilized probe. probe typically via magnetic bead. amplified thermocycling without prior dissociation beads. efficiency method lies part that both steps performed single vessel. can then be sequenced.