Hydrogen production by Rhodobacter sphaeroides strain O.U.001 using spent media of Enterobacter cloacae strain DM11

摘要: Combined dark and photo-fermentation was carried out to study the feasibility of biological hydrogen production. In fermentation, produced by Enterobacter cloacae strain DM11 using glucose as substrate. This followed a process. Here, spent medium from process (containing unconverted metabolites, mainly acetic acid etc.) underwent Rhodobacter sphaeroides O.U.001 in column photo-bioreactor. combination could achieve higher yields complete utilization chemical energy stored Dark fermentation studied terms several parameters, such initial substrate concentration, pH temperature, establish favorable conditions for maximum Also, effects threshold concentration acid, light intensity presence additional nitrogen sources effluent on amount during were investigated. The conversion efficiency found be inversely proportional incident intensity. batch system, yield about 1.86 mol H2 mol−1 glucose; 1.5–1.72 acid. overall combined process, considering preliminary substrate, than that single