Enhancing fatty acid ethyl ester production in Saccharomyces cerevisiae through metabolic engineering and medium optimization.
作者： R. Adam Thompson , Cong T. Trinh
DOI: 10.1002/BIT.25292
关键词:
摘要: Biodiesels in the form of fatty acyl ethyl esters (FAEEs) are a promising next generation biofuel due to their chemical properties and compatibility with existing infrastructure. It has recently been shown that expression bacterial acyl-transferase established industrial workhorse Saccharomyces cerevisiae can lead production FAEEs by condensation acyl-CoAs ethanol. In contrast recent strategies produce S. through manipulation de novo acid biosynthesis or series arduous genetic manipulations, we introduced novel background, which is comparable titer previous reports fraction disruption aiming at increasing acyl-CoA pools. addition, combined metabolic engineering modification culture conditions maximum over 25 mg/L FAEEs, 40% improvement 17-fold our initial characterizations. Biotechnol. Bioeng. 2014;111: 2200-2208. © 2014 Wiley Periodicals, Inc.
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