Erythropoietin enhances cell proliferation and survival of human fetal neuronal progenitors in normoxia

摘要: Abstract Extensive data reporting the neurogenerative, neuroprotective and neuroregenerative potential of erythropoietin (EPO), mainly on RNA level, can be found in literature. However, there is still a poor knowledge response neuronal progenitor cells (NPC) upon stimulation with EPO terms protein species involved. Herein, effect proliferation human mesencephalic NPC (hmNPC) under normoxia monitored using cellular assays proteomic analysis (two-dimensional gel electrophoresis MALDI-TOF mass spectrometry). The administration increased hmNPC within 4 days after application. It positively influenced cell-cycle progression by affecting G2 phase cell cycle. A expression cultures treatment identified 8 proteins differentially expressed EPO-treated cultures. likely that one or more are involved pathways promote differentiation normoxia. Their further characterization could provide targets for development new therapeutic agents to treat CNS injury. Moreover, as signaling hypoxia-inducible, our findings may also indicate beneficial mimic hypoxia, while bypassing its negative effects, culture fetal midbrain-derived cells.