[14C]Guanidinium ion influx into Na+ channel preparations from mouse cerebral cortex
作者： Maarten E.A. Reith
DOI: 10.1016/0922-4106(90)90245-S
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摘要: Abstract [14C]Guanidinium ion influx into Na+ channel preparations from mouse and rat cerebral cortex (purified synaptosomes, synaptoneurosomes) was characterized its properties were compared with those for 22Na+ influx. Tetrodotoxin-sensitive of [14C]guanidinium stimulated by aconitine, veratridine, batrachotoxin a K0.5 7, 5 0.3 μM, respectively, the maximal being same all toxins. Scorpion venom shifted activation curve veratridine to left, but did not increase The potency local anesthetic drugs cocaine tetracaine in inhibiting depended upon concentration used activate channels. mechanism inhibition competitive nature. Other congeners inhibited potencies very similar results show that is valid model through voltage-dependent channels although there are some differences between two assays. guanidinium assay offers convenience 14C isotope as strongly radiating isotope.
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