Magnetic resonance imaging of gadolinium-labeled pancreatic islets for experimental transplantation
作者： Luigi Biancone , Simonetta Geninatti Crich , Vincenzo Cantaluppi , Giuseppe Mauriello Romanazzi , Simona Russo
DOI: 10.1002/NBM.1088
关键词:
摘要: New imaging techniques that couple anatomical resolution to sensitivity may greatly contribute improving islet transplantation. In the present work, a report is given of direct detection islets by magnetic resonance (MRI) after ex vivo cell labeling with MRI T1 contrast agent GdHPDO3A. Experiments on mouse and human demonstrated well-tolerated uptake GdHPDO3A, based morphology, viability, glucose-dependent insulin response apoptosis/toxicity gene array profile. GdHPDO3A loading was sufficient for in vitro detection. isotransplanted into kidney capsule xenotransplanted within liver were detected. Imaging specificity supported absence signal unlabeled transplants, its persistence upon using fat-suppression protocols colocalization transplanted islets. conclusion, high spatial demonstrated, allowing visualization subcapsular grafts intrahepatic xenografts. Copyright © 2006 John Wiley & Sons, Ltd.
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