Registration of 'NIBGE-2' cotton

摘要: M. Rahman and Y. Zafar, Plant Genomics & Mol Breeding (PGMB) Labs., National Institute for Biotechnology Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road, Faisalabad, Pakistan. Registration by CSSA. Received 10 Dec. 2006. *Corresponding author (mehboob@nibge.org or mehboob_pbd@yahoo.com). NIBGE-115 cotton (Gossypium hirsutum L.) (Reg. No. GP-880, PI 643972) was developed in 2002 the Molecular Labs, Pakistan, released It bred as part of an ongoing effort to develop cultivars with improved resistance a breaking strain leaf curl disease (CLCuD; transmitted whitefl y Bemisia tabaci Genn.) called Burewala (Briddon, 2003). The original CLCuD is caused one (or more) seven begomoviruses distinct DNA satellite (Briddon et al., 2001; Briddon, (Burewala strain) has yet be fully characterized but associated recombinant (Amin 2006). pedigree selection from cross (made 1997–1998) unreleased genotype LRA-5166 (primary source old CLCuD) ‘S-12’ (highly susceptible CLCuD). F2 plant along spreader rows highly S-12 (every third row) were sown last week June 1998 at NIBGE fi eld ensure maximum infection (Rahman 2002). No control measures implemented, thus allowing spread vector populations throughout season. plants succeeding generations screened using three procedures: evaluation, transmission assay, graft inoculation Viral determined visual symptom assessment well dot-blot multiplex polymerase chain reaction (PCR) diagnostic techniques 2005). Greenhouse grown F3 progeny resistant raised PCR tests. Resistant advanced F4 again subjected screening tests 1999. procedure repeated F7. These lines given acronym after institute’s name, Engineering, 2002. In normal growing season 2002–2003, inbred region (Cotton Research Station, Vehari, Pakistan) provide inoculum CLCuD. Out these, found virus free 2004; Rezian, 2003–2004, genotypes strain, both visually PCR-based test A standard randomized complete block design four replications followed. continued two successive cotton-growing seasons (2004– 20 genotypes, showed against nectaried, possesses normal-shaped leaves bracts pubescent stems leaves, glanded, exhibiting spreading type habit 0 4 monopodial branches. Flowering initiates 40 47 d planting pollen being cream colored. Average lint yields 7.48% more than CIM-496 (standard) all locations conducted 2004 Fiber properties line (measured high volume instrument) fairly acceptable (3% shorter, 5% stronger, higher micronaire value that CIM-496). germplasm will useful breeding disease. Limited quantity seed distributed on written request corresponding author. receiving agency entrepreneur asked make appropriate recognition if it contributes developing hybrids, new cultivars, lines.