Characterization of Muscle Sarcoplasmic and Myofibrillar Protein Hydrolysis Caused by Lactobacillus plantarum

摘要: Strains of Lactobacillus plantarum originally isolated from sausages were screened for proteinase and aminopeptidase activities toward synthetic substrates; on the basis that screening, L. CRL 681 was selected further assays muscle proteins. The whole cells, cell extracts (CE), a combination both sarcoplasmic myofibrillar protein determined by protein, peptide, free-amino-acid analyses. Proteinase cells initiated hydrolysis addition CE intensified proteolysis. Whole generated hydrophilic peptides Other hydrophobic nature resulted CE. action enzymatic sources proteins caused maximal increases in lysine, arginine, leucine, while those mainly released alanine. In general, pronounced required enzyme to supplied