Quantifying the influences of radiation therapy on deformability of human red blood cells by dual-beam optical tweezers

摘要: Radiation therapy is widely used as a treatment tool for malignancies. However, radiation-related complications are still unavoidable risks off-target cells. Little known about radiation therapy's possible effects on mechanical features of the cells such human red blood (RBCs). RBCs nucleus-free circulating that can deform without losing functionality in healthy conditions. Thus, to evaluate vitro plasma membrane cells, were selected primary test model. exposed clinically prescribed radiotherapy doses 2 Gy, 12 Gy and, 25 and each dose group was compared non-irradiated group. Cells characterized by stretching using dual-beam optical tweezers resulting deformability index. The receiving highest found statistically distinguishable from control (DI0Gy = 0.33 ± 0.08), revealed index (DI25Gy 0.38 0.11, p 0.0068), while no significant differences (DI2Gy 0.08, 0.9) (DI12Gy 0.31 0.09, 0.2) groups. Based these findings, we conclude exposure may alter depending amount, measurement serve sensitive biomarker probe responses radiotherapy.