Uptake of neoglycoproteins via membrane lectin(s) of L1210 cells evidenced by quantitative flow cytofluorometry and drug targeting

摘要: The presence and the sugar specificity of membrane lectins on cell surface mouse L1210 leukemia cells were demonstrated by using various neoglycoproteins (glycosylated serum albumin) substituted with fluorescein or methotrexate. Neoglycoproteins prepared reaction glycosidophenylisothiocyanates bovine albumin. binding to depends nature carried number bound residues per neoglycoprotein molecule. best results obtained fucosylated albumin containing 25 +/- 5 fucose. amount cell-associated fluorescein-labeled was several fold higher at 37 degrees C than 4 suggesting a specific endocytotic process. lectin-mediated endocytosis further showing that fluorescence upon incubation in increased after addition monensin, proton/sodium ionophore known raise pH endosomes lysosomes. analysis association achieved quantitative flow cytofluorometry standardization calibrated polystyrene sulfonate beads carrying amounts 1-(fluoresceinylthioureido)-4,8-diazalicosane. In addition, cytotoxicity neoglycoprotein-bound methotrexate shown be related sugar: most efficient carrier, have close anti IgM monoclonal antibody