LncRNA RMST-mediated miR-107 transcription promotes OGD-induced neuronal apoptosis via interacting with hnRNPK
作者： Hong Cheng , Mei Sun , Zhao-Lu Wang , Qian Wu , Juan Yao
DOI: 10.1016/J.NEUINT.2019.104644
关键词:
摘要: Abstract The long noncoding RNA (lncRNA) rhabdomyosarcoma 2-associated transcript (RMST) silencing has been demonstrated to protect against ischemic brain injury in vivo and neuron vitro. However, its underlying mechanisms the progression of stroke have not well explored. expression RMST oxygen-glucose deprivation (OGD)-treated HT-22 hippocampal cell line was examined using quantitative Real-Time PCR (qRT-PCR). CCK-8 cell viability apoptotic detection Annexin V-FITC PI staining coupled with flow cytometry were performed determine pro-apoptotic role line. Furthermore, pull-down, immunoprecipitation (RIP), coimmunoprecipitation (Co-IP), chromatin (ChIP) dual-Luciferase reporter assays mechanism OGD-induced HT-22 cell apoptosis. In results, highly expressed OGD-treated HT-22 cells. Altered led marked changes proliferation Mechanistically, indirectly activated p53/miR-107 signaling pathway via interacting heterogeneous nuclear ribonucleoprotein K (hnRNPK) fulfilled function conclusion, our data indicated that RMST/hnRNPK/p53/miR-107/Bcl2l2 axis plays an important regulating neuronal
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