Intracellular Site of Synthesis of Microsomal Heme Oxygenase in Pig Spleen

摘要: In the pig spleen specific activity of heme oxygenase was two to three times higher in smooth microsomes than rough microsomes, whereas total activities recovered microsomal fractions were similar. Free and bound polysomes isolated from nascent peptides on these analyzed by employing [3H]puromycin a oxygenase-specific rabbit antibody (IgG). It shown that free are major site synthesis. addition, cell-free synthesis performed reticulocyte lysate system with spleen, results obtained again indicated is synthesized predominantly polysomes. The newly may be incorporated first into portion endoplasmic reticulum either before or after its release polysomes, although this enzyme at steady state considerably region.