Neuronal cell surfaces: distinctive glycoproteins of cultured adrenergic and cholinergic sympathetic neurons

摘要: Cell surface components which are candidates for a role in nerve-target interactions specific neurons of particular transmitter types the sympathetic nervous system have been identified. Neurons superior cervical ganglia neonatal rats were dissociated and cultured virtual absence non-neuronal cells under conditions previously found to control their choice neurotransmitter. When raised medium conditioned by heart cells, become cholinergic; when depolarizes them, remain original adrenergic state. The cell proteins labeled either metabolic or surface-specific methods, separated two-dimensional polyacrylamide gel electrophoresis, visualized autoradiography. A total approximately 35 glycoproteins can be resolved, at least 14 exposed on surface. We evaluated differ potency inducing cholinergic properties: skeletal muscle, liver, containing 1 mM butyric acid. expression three neuronal was correlated with ability given culture condition induce synthesis accumulation acetylcholine catecholamines. Two these surface, third appears identical protein shown secreted into medium.