TR-NOE and MD studies ofLeishmania gp63 SRYD-containing sequences bound to anti-SRYD monoclonal antibody
作者： Vassilios Tsikaris , Marie-Christine Petit , Piotr Orlewski , Maria Sakarellos-Daitsiotis , Constantinos Sakarellos
DOI: 10.1007/BF02442896
关键词:
摘要: The I250 ASRYDQL257 synthetic octapeptide of theLeishmania major surface glycoprotein gp63, which efficiently inhibits parasite attachment to the macrophage receptors and mimics antigenically functionally RGDS sequence fibronectin, was studied by 2D TR-NOESY in presence an anti-SRYD monoclonal antibody (mAbSRYD) that recognizes both SRYD-containing peptides cognate protein on intact parasites. Molecular modeling performed using distance constraints obtained from TR-NOEs. bound structure compared with free peptide DMSO solution crystal RYD fragment OPG2 Fab, antireceptor RGD cell adhesion site.
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sci-hub.se 参考文章(21)
Kurt Wuthrich, NMR of proteins and nucleic acids ,(1986)
K.P. Soteriadou, M.S. Remoundos, M.C. Katsikas, A.K. Tzinia, V Tsikaris, C Sakarellos, S.J. Tzartos, The Ser-Arg-Tyr-Asp region of the major surface glycoprotein of Leishmania mimics the Arg-Gly-Asp-Ser cell attachment region of fibronectin. Journal of Biological Chemistry. ,vol. 267, pp. 13980- 13985 ,(1992) , 10.1016/S0021-9258(19)49666-X
D M Mosser, A Brittingham, C J Morrison, B S McGwire, W R McMaster, K P Chang, Role of the Leishmania surface protease gp63 in complement fixation, cell adhesion, and resistance to complement-mediated lysis. Journal of Immunology. ,vol. 155, pp. 3102- 3111 ,(1995)
Ramadurgam Kodandapani, B. Veerapandian, Thomas J. Kunicki, Kathryn R. Ely, Crystal Structure of the OPG2 Fab AN ANTIRECEPTOR ANTIBODY THAT MIMICS AN RGD CELL ADHESION SITE Journal of Biological Chemistry. ,vol. 270, pp. 2268- 2273 ,(1995) , 10.1074/JBC.270.5.2268
K P Soteriadou, A K Tzinia, M G Hadziantoniou, S J Tzartos, Identification of monomeric and oligomeric forms of a major Leishmania infantum antigen by using monoclonal antibodies Infection and Immunity. ,vol. 56, pp. 1180- 1186 ,(1988) , 10.1128/IAI.56.5.1180-1186.1988
Peter Güntert, Werner Braun, Kurt Wüthrich, Efficient computation of three-dimensional protein structures in solution from nuclear magnetic resonance data using the program DIANA and the supporting programs CALIBA, HABAS and GLOMSA. Journal of Molecular Biology. ,vol. 217, pp. 517- 530 ,(1991) , 10.1016/0022-2836(91)90754-T
Timothy A. Springer, Adhesion receptors of the immune system. Nature. ,vol. 346, pp. 425- 434 ,(1990) , 10.1038/346425A0
C. Bordier, The promastigote surface protease of leishmania Parasitology Today. ,vol. 3, pp. 151- 153 ,(1987) , 10.1016/0169-4758(87)90199-2
Anil Kumar, Gerhard Wagner, Richard R. Ernst, Kurt Wuethrich, Buildup rates of the nuclear Overhauser effect measured by two-dimensional proton magnetic resonance spectroscopy: implications for studies of protein conformation Journal of the American Chemical Society. ,vol. 103, pp. 3654- 3658 ,(1981) , 10.1021/JA00403A008
Martial Piotto, Vladimir Saudek, Vladimir Sklenář, Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions. Journal of Biomolecular NMR. ,vol. 2, pp. 661- 665 ,(1992) , 10.1007/BF02192855