Effect of oxygen concentration and selected protocol factors on viability and gene expression of mouse liver slices.

摘要: Abstract Precision cut liver slices (PCLSs) are widely used as a model to study hepatotoxicity. For culturing of PCLS diverse protocols which could affect viability and results. We aimed identify the most optimal culture protocol for mouse PCLS. Slices were cultured 24 h under different concentrations serum, glucose, insulin, oxygen. Thereafter, was assessed by biochemical methods. Transcriptome analysis performed changes introduced at oxygen (20%, 40%, 60%, 80% oxygen). Medium composition did not viability. Although metabolic competence unaffected concentrations, yielded with best characteristics. The comparison uncultured vs. revealed 2524 genes be differentially expressed. Genes involved in drug metabolism, peroxisomal mitochondrial functions down-regulated while several adaptive/stress response processes up-regulated. Moreover, favorable condition respect maintenance expression energy metabolism. outcome this indicates that valuable tool research on hepatic toxicity, particularly if they controlled concentration 80%.