L-Threonine dehydrogenase from goat liver. Feedback inhibition by methylglyoxal.
作者： M Ray , S Ray
DOI: 10.1016/S0021-9258(18)88915-3
关键词:
摘要: L-Threonine dehydrogenase, which forms aminoacetone from L-threonine and NAD, has been extensively purified goat liver. A feedback inhibition of this enzyme observed with methylglyoxal. Kinetic data other experiments indicate that methylglyoxal acts at a site than the active enzyme. The contains single subunit Mr 89,000. apparent Km values for NAD were found to be 5.5 1 mM, respectively.
sci-hub.st 参考文章(20)
M Ray, S Ray, On the interaction of nucleotides and glycolytic intermediates with NAD-linked alpha-ketoaldehyde dehydrogenase. Journal of Biological Chemistry. ,vol. 257, pp. 10571- 10574 ,(1982) , 10.1016/S0021-9258(18)33859-6
M I Bird, P B Nunn, Metabolic homoeostasis of L-threonine in the normally-fed rat. Importance of liver threonine dehydrogenase activity. Biochemical Journal. ,vol. 214, pp. 687- 694 ,(1983) , 10.1042/BJ2140687
D. McGilvray, J G Morris, Utilization of L-threonine by a species of Arthrobacter. A novel catabolic role for "aminoacetone synthase". Biochemical Journal. ,vol. 112, pp. 657- 671 ,(1969) , 10.1042/BJ1120657
Gumpei Urata, S. Granick, Biosynthesis of α-Aminoketones and the Metabolism of Aminoacetone Journal of Biological Chemistry. ,vol. 238, pp. 811- 820 ,(1963) , 10.1016/S0021-9258(18)81339-4
S.A. Boylan, E.E. Dekker, L-threonine dehydrogenase. Purification and properties of the homogeneous enzyme from Escherichia coli K-12. Journal of Biological Chemistry. ,vol. 256, pp. 1809- 1815 ,(1981) , 10.1016/S0021-9258(19)69880-7
D. J. Hopper, R. A. Cooper, The purification and properties of Escherichia coli methylglyoxal synthase. Biochemical Journal. ,vol. 128, pp. 321- 329 ,(1972) , 10.1042/BJ1280321
S. Ray, M. Ray, Isolation of methylglyoxal synthase from goat liver. Journal of Biological Chemistry. ,vol. 256, pp. 6230- 6233 ,(1981) , 10.1016/S0021-9258(19)69151-9
S Ray, M Ray, Formation of methylglyoxal from aminoacetone by amine oxidase from goat plasma. Journal of Biological Chemistry. ,vol. 258, pp. 3461- 3462 ,(1983) , 10.1016/S0021-9258(18)32683-8
Y. Aoyama, Y. Motokawa, L-Threonine dehydrogenase of chicken liver. Purification, characterization, and physiological significance. Journal of Biological Chemistry. ,vol. 256, pp. 12367- 12373 ,(1981) , 10.1016/S0021-9258(18)43281-4
R.A. Cooper, [104] Methylglyoxal synthase Methods in Enzymology. ,vol. 41, pp. 502- 508 ,(1975) , 10.1016/S0076-6879(75)41106-5