The effect of serum on the transport and phosphorylation of 2-deoxyglucose by untransformed and transformed mouse 3T3 cells
作者： John A. Hassell , Clarence Colby , Antonio H. Romano
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摘要: Serum starvation of growing and nongrowing (density-inhibited) mouse 3T3 cells resulted in decreased phosphorylation 2-deoxy--D-glucose, while the time course transport this sugar remained unchanged. SV40 transformed (SV101) spontaneously 3T6 did not alter either transport, or sugar. Treatment SV101 with 10(-4) M dibutyryl adenosine cyclic 3':5' monophosphate 10(-3) theophylline restore capacity to regulate 2-deoxy-D-glucose when these were serum deprived. We conclude that factors are involved modulation rather than its transport. This regulation is operative both as well cells. In contrast, do respond phosphorylation.
sci-hub.se 参考文章(28)
Rolf F. Kletzien, James F. Perdue, Sugar transport in chick embryo fibroblasts. I. A functional change in the plasma membrane associated with the rate of cell growth. Journal of Biological Chemistry. ,vol. 249, pp. 3366- 3374 ,(1974) , 10.1016/S0021-9258(19)42581-7
Wayne L. Ryan, Margaret L. Heidrick, Cyclic nucleotides on cell growth in vitro. Cancer Research. ,vol. 30, pp. 376- 378 ,(1970)
Adele R. Schultz, Lloyd A. Culp, Contact-inhibited revertant cell lines isolated from SV40-transformed cells. V. Contact inhibition of sugar transport. Experimental Cell Research. ,vol. 81, pp. 95- 103 ,(1973) , 10.1016/0014-4827(73)90115-8
D O Foster, A B Pardee, Transport of amino acids by confluent and nonconfluent 3T3 and polyoma virus-transformed 3T3 cells growing on glass cover slips. Journal of Biological Chemistry. ,vol. 244, pp. 2675- 2681 ,(1969) , 10.1016/S0021-9258(18)83451-2
BENJAMIN LEWIN, Cells in Charge Nature. ,vol. 232, pp. 206- 206 ,(1971) , 10.1038/232206A0
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
R. Kram, G. M. Tomkins, Pleiotypic Control by Cyclic AMP: Interaction with Cyclic GMP and Possible Role of Microtubules Proceedings of the National Academy of Sciences of the United States of America. ,vol. 70, pp. 1659- 1663 ,(1973) , 10.1073/PNAS.70.6.1659
George J. Todaro, Gerald K. Lazar, Howard Green, The initiation of cell division in a contact-inhibited mammalian cell line Journal of Cellular and Comparative Physiology. ,vol. 66, pp. 325- 333 ,(1965) , 10.1002/JCP.1030660310
C. Colby, A. H. Romano, Phosphorylation but not transport of sugars is enhanced in virus-transformed mouse 3T3 cells Journal of Cellular Physiology. ,vol. 85, pp. 15- 23 ,(1975) , 10.1002/JCP.1040850103
S. K. Bose, B. J. Zlotnick, Growth- and Density-Dependent Inhibition of Deoxyglucose Transport in Balb 3T3 Cells and Its Absence in Cells Transformed by Murine Sarcoma Virus Proceedings of the National Academy of Sciences of the United States of America. ,vol. 70, pp. 2374- 2378 ,(1973) , 10.1073/PNAS.70.8.2374