Characterization of an antibacterial factor in human parotid secretions, active against Lactobacillus casei

摘要: Further characteristics of an antibacterial agent present in human parotid secretion, active against Lactobacillus casei (A.T.C.C. 4646), have been demonstrated. Two components, consisting a salivary protein fraction and thiocyanate, are necessary for the inhibiting activity secretion. The appears to possess enzyme, whereas thiocyanate acts as substrate rather than co-factor. Fractional extraction test organism has yielded acceptor material located cytoplasm associated with cell wall. sulphur carbon atoms ion transferred material. Thiocyanate reaction bacterial component presence non-diffusable was demonstrated both by decrease original medium ionic appearance reacted within inhibited cells. complex shown altered charge exchange behaviour molecular weight under 5000 determined on Sephadex gel columns. bulk detected growth cells its behavior