Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis

摘要: We describe ten new species of treefrogs assigned to the genus Boophis (Anura: Mantellidae) and resurrect two from synonymy, based on materials collected during fieldwork in Madagascar, carried out mainly between 2000 2007. Our comparative database assembled over past years comprises fresh material for molecular analysis all 58 nominal species, advertisement call recordings except three species. follow an integrative approach combine molecular, bioacoustic morphological evidence diagnose In most cases, have uncorrected divergences 4–5% mitochondrial 16S rRNA gene their closest relatives. some cases are lower (2–2.5%) but then accompanied by distinct differences calls or morphology. piperatus sp. nov. Ranomafana National Park is a small brown B. majori group that similar miniatus differs morphology calls. arcanus as well known only female specimens site close Ranomafana; it described its strong genetic differentiation (> 7.2% other species). entingae goudoti occurring northern sympatric with brachychir, strongly different call. roseipalmatus belongs group, madagascariensis, appears replace this possible areas sympatry north east. spinophis enigmatic, morphologically highly divergent into spe-4 · Zootaxa 2383 © 2010 Magnolia Presscies having dermal tubercles along lateral parts shank around elbow. praedictus sibling albilabris diagnosable red iris periphery distributed rainforest east coast. sandrae luteus superficially elenae, has faster smaller body size. miadana haingana nov., both albipunctatus syntopically at Andohahela Park, related ankaratra schuboeae differ luciae also sibilans slightly size furthermore Rhacophorus obscurus Boettger, 1913 (as group) synonymy andrangoloaka Ahl, 1928 microtympanum rhodoscelis, propose consider brevirostris junior synonym andrangoloaka. discuss our methodological lines used delimitate resurrected herein. By applying IUCN Redlist criteria, we evaluate threat status considered: six classified Data Deficient (B. arcanus, haingana, miadana, piperatus, praedictus, spinophis), four Vulnerable andrangoloaka, entingae, roseipalmatus, sandrae), Least Concern luciae, obscurus).