Impact of non-thermal plasma treatment on MAPK signaling pathways of human immune cell lines.

摘要: Abstract In the field of wound healing research non-thermal plasma (NTP) increasingly draws attention. Next to its intensely studied antibacterial effects, some studies already showed stimulating effects on eukaryotic cells. This promises a unique potential in chronic wounds, where effective therapies are urgently needed. Immune cells do play an important part process and their reaction NTP treatment has yet been rarely examined. Here, we impact using kinpen apoptotic proliferative cell signaling pathways two human immune lines, CD4 + T helper line Jurkat monocyte THP-1. Depending time number increased both investigated types according caspase 3 assay. Western blot analysis pointed out that activated pro-apoptotic proteins like p38 mitogen-activated protein kinase (p38 MAPK) c-Jun N-terminal 1 2 (JNK 1/2) types. Stronger signals were detected at comparable times. Intriguingly, exposure THP-1 also pro-proliferative molecules extracellular signal-regulated 1/2 (ERK MAPK/ERK (MEK 1/2). contrast cells, anti-apoptotic heat shock 27 (HSP27) was after treatment, indicating possible mechanism how may reduce programmed death. conclusion, several cascades examined lines monocytes defense against impacts could be revealed. Therefore, might option for healing.