Higher Concentrations of Heparin and Hirudin Are Required to Inhibit Thrombin Generation in Tissue Factor–Activated Cord Plasma Than in Adult Plasma

摘要: In standard clotting assays plasma samples are markedly diluted and high amounts of initiator added to trigger clot formation. Under these conditions, adult clots faster than neonatal only 30–50% peak thrombin activity can be produced in plasma. It was reported that decreased concentrations heparinoids required inhibit generation from newborns the same extend as adults due reduced potential (TP). Plasma activation by addition low lipidated tissue factor (TF) combination with slight dilution probably better reflects conditions vivo allows sensitive detection effects anticoagulants on generation. We have shown recently under cord higher generated compared accordance, we show present study heparin hirudin for effective inhibition plasma: Prolongation time suppression TP were significantly more pronounced coagulant challenge contrast, prolongation challenge. Our results suggest neonates very much depend type used initiate doses not derived studies done adults, inhibitors may required.