Challenges in the transition from in vitro to in vivo experiments: source effects and biological aspects

摘要: The antitumor action of Low Temperature Non Thermal Plasma (LTNTP) has been now widely demonstrated both in vitro and vivo (on animal models more recently on human [1]) a large variety cancer cell lines tumor types. Nevertheless, due to the great plasma components able play role, precise understanding process chain leading observed effects is far be reached. It not easy isolate or measure component individual or, contrast, evaluate potential benefit synergy combined ones. Up now, many experiments have realized with that intention, but translation results rather complicated, even, cases, impossible complex environment cells vivo, including vascularization (then blood flow oxygenation/physioxia) body response (signaling, recruitment, immune system). Generally, first step dedicated studies concerns viability toxicity culture media analysis induced changes medium. must stressed used experimental conditions together fact treat multiwell plates outside their incubation chamber may generate stress can affect behavior then biased results. This presentation will show some issues challenges associated cancerous treatments by jet (here Gun), also relation hypoxic/physioxic environment. We present direct effect treatment medium cells, liquid evaporation, oxygen deprivation, extra intracellular RONS generation, membrane poration, depolarization, role electric field treated adjacent wells [2]. We particularly emphasize importance oxygenation level which key factor proliferation/regression tumors hypoxic environment. shown an uncontrolled lead drastic pressure (and temperature) during experiment sequences maxima (leading normoxia) minima (potentially strong hypoxia). latter contrasts increases measured [3]. means attention paid modeling interactions jets physiological since initial concentration greatly affected. Acknowledgments This work founded ARD 2020 Cosmetoscience PLASMACOSM project. X.D. supported INEL/Region Centre-Val de Loire fellowship. References [1] Metelmann et al, Clinical Medicine, 3 17-23 (2015). [2] E. Robert, T. Darny, S. Dozias, Iseni, J. M. Pouvesle, Phys. Plasmas 22, 122007 (2015). [3] G. Collet, A. Lenoir, Vandamme, C. Kieda, Sources Sci. Technol.23, 012005 (2014).