Potential utility of HOP homeobox gene promoter methylation as a marker of tumor aggressiveness in gastric cancer.

摘要: HOP homeobox (HOPX) is an unusual gene encoding three spliced transcript variants, among which the only HOPX-β promoter harbors CpG islands. The characteristics of its methylation was analyzed using bisulfite sequencing and quantitative-methylation-specific polymerase chain reaction (Q-MSP), effects HOPX expression were also examined. silenced in all gastric cancer cell lines tested; could be restored by treatment with demethylating agent. On Q-MSP, hypermethylation (cut-off value 3.55) found 84% (67 out 80) primary tumor tissues 10% (8 corresponding normal discriminate from (P<0.0001). prognosis advanced cases as poor those stage IV disease when cut-off set at 11.28. This finding validated independent cohort 90 cancers. prognostic factor (P=0.029) on multivariate analysis. Exogenous significantly inhibited proliferation, colony formation invasion well enhanced apoptosis. Taken together, a frequent cancer-specific event cancer. Quantitative assessment has great clinical potential marker aggressiveness.