Dynamics of Metal Partitioning at the Cell–Solution Interface: Implications for Toxicity Assessment under Growth-Inhibiting Conditions

摘要: Metal toxicity toward microorganisms is usually evaluated by determining growth inhibition. To achieve a mechanistic interpretation of such toxic effects, the intricate coupling between cell kinetics and metal partitioning dynamics at cell-solution interface over time must be considered on quantitative level. A formalism elaborated to evaluate cell-surface-bound, internalized, extracellular fractions in limit where uptake controlled internalization under noncomplexing medium conditions. Cell tackled using continuous logistic equation modified include inhibition accumulation intracellular or surface sites. The theory further includes metal-proton competition for adsorption cell-surface binding sites, as well possible variation size during exposure ions. elucidates dramatic impacts initial concentration bioavailability time, agreement with reported algae bioassays. It highlights that appropriate definition endpoints requires careful inspection ratio scale depletion from bulk solution. latter depends internalization-excretion rate constants, microorganism growth, extent nonspecific, transporter, inhibitory As an application theory, Cd Pseudokirchneriella subcapitata interpreted constrained modeling interfacial Cd-partitioning measured various