Structural features of ribonucleotide reductase

作者: Ioannis Nikas , John McLauchlan , Andrew J. Davison , William R. Taylor , J. Barklie Clements

DOI: 10.1002/PROT.340010411

关键词:

摘要: Herpes simplex virus type 1 (HSV-1) encodes a ribonucleotide reductase which comprises two polypeptides with sizes of 136,000 (RR1) and 38,000 mol. wt. (RR2). We have determined the entire DNA sequence specifying HSV-1 RR1 identified adjacent open reading frames in varicella-zoster (VZV) homology to HSV RR2; predicted for VZV RR2 are 87,000 35,000 respectively. Amino acid comparisons from other organisms indicate that contains unique N-terminal domain is absent apart HSV-2 RR1. These amino sequences poorly conserved between contrast remainder protein shows greater than 90% homology. Polypeptide structural predictions suggest may be separated into regions, namely, beta-sheet structure followed by nonstructured area. Across RR2, also reveal blocks acids different reductases, these important enzyme activity. From on blocks, we proposed location substrate binding site within centered three glycine residues region adopt beta-sheet/turn/alpha-helical structure; this approximates ADP nucleotide folds. Finally, propose promoters Epstein-Barr (EBV) transcripts evolved separate evolutionary routes.

参考文章(49)
U von Döbeln, P Reichard, Binding of substrates to Escherichia coli ribonucleotide reductase. Journal of Biological Chemistry. ,vol. 251, pp. 3616- 3622 ,(1976) , 10.1016/S0021-9258(17)33389-6
I W Caras, B B Levinson, M Fabry, S R Williams, D W Martin, Cloned mouse ribonucleotide reductase subunit M1 cDNA reveals amino acid sequence homology with Escherichia coli and herpesvirus ribonucleotide reductases. Journal of Biological Chemistry. ,vol. 260, pp. 7015- 7022 ,(1985) , 10.1016/S0021-9258(18)88881-0
H S Marsden, N D Stow, V G Preston, M C Timbury, N M Wilkie, Physical mapping of herpes simplex virus-induced polypeptides. Journal of Virology. ,vol. 28, pp. 624- 642 ,(1978) , 10.1128/JVI.28.2.624-642.1978
M Carlquist, B M Sjöberg, H Jörnvall, S Eriksson, A photoaffinity-labeled allosteric site in Escherichia coli ribonucleotide reductase. Journal of Biological Chemistry. ,vol. 261, pp. 1878- 1882 ,(1986) , 10.1016/S0021-9258(17)36024-6
Gary H. Cohen, Mindie N. Factor, Manuel Ponce de Leon, Inhibition of Herpes Simplex Virus Type 2 Replication by Thymidine Journal of Virology. ,vol. 14, pp. 20- 25 ,(1974) , 10.1128/JVI.14.1.20-25.1974
D R Averett, C Lubbers, G B Elion, T Spector, Ribonucleotide reductase induced by herpes simplex type 1 virus. Characterization of a distinct enzyme. Journal of Biological Chemistry. ,vol. 258, pp. 9831- 9838 ,(1983) , 10.1016/S0021-9258(17)44573-X
B.M. Sjöberg, S. Hahne, C.Z. Mathews, C.K. Mathews, K.N. Rand, M.J. Gait, The bacteriophage T4 gene for the small subunit of ribonucleotide reductase contains an intron. The EMBO Journal. ,vol. 5, pp. 2031- 2036 ,(1986) , 10.1002/J.1460-2075.1986.TB04460.X