ANALYSIS OF EXPRESSED SEQUENCE TAGS FROM THE GREEN ALGA ULVA LINZA (CHLOROPHYTA) 1

摘要: There is a general lack of genomic information available for chlorophyte seaweed genera such as Ulva, and in particular there no concerning the genes that contribute to adhesion cell wall biosynthesis this organism. Partial sequencing cDNA libraries generate expressed sequence tags (ESTs) an effective means gene discovery characterization expression patterns. In study, library was created from sporulating tissue Ulva linza L. Initially, 650 ESTs were randomly selected sequenced their 5 0 ends obtain indication level redundancy (21%). The normalized enrich rarer sequences, further 1920 sequenced. These sequences subjected contig assembly resulted unigene set approximately 1104 ESTs. Forty-eight percent these exhibited significant similarity databases. Phylogenetic comparisons are made between with databases proteins involved aspects extracellular matrix/cell adhesion.