IL-10 and Natural Regulatory T Cells: Two Independent Anti-Inflammatory Mechanisms in Herpes Simplex Virus-Induced Ocular Immunopathology

作者: Pranita P. Sarangi , Sharvan Sehrawat , Susmit Suvas , Barry T. Rouse

DOI: 10.4049/JIMMUNOL.180.9.6297

关键词:

摘要: Two prominent anti-inflammatory mechanisms involved in controlling HSV-1-induced corneal immunopathology (stromal keratitis or SK) are the production of cytokine IL-10 and activity natural regulatory T cells (nTregs). It is not known whether, under vivo conditions, nTregs influence pathology independently concert. In current study using wild-type −/− animals, we have assessed absence both vitro conditions. The animals depleted before ocular infection showed more severe SK lesions as compared with undepleted animals. addition, purified from naive WT were equally able to suppress proliferation anti-CD3-stimulated CD4 + CD25 − vitro. Furthermore, intracellular staining results indicated that nonregulatory expressing B220 markers major IL-10-producing cell types lymphoid tissues HSV-infected mice. contrast, infected corneas, CD11b Gr1 phenotype along a minor population Foxp3 few F4/80 produced IL-10. Our investigations indicate at least two independent limiting SK, which may need be modulated control therapeutically.

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