The gene encoding polyneuridine aldehyde esterase of monoterpenoid indole alkaloid biosynthesis in plants is an ortholog of theα/β hydrolase super family
作者： Emine Dogru , Heribert Warzecha , Frank Seibel , Sophie Haebel , Friedrich Lottspeich
DOI: 10.1046/J.1432-1327.2000.01136.X
关键词:
摘要: The biosynthesis of the anti-arrhythmic alkaloid ajmaline is catalysed by more than 10 specific enzymes. In this multistep process polyneuridine aldehyde esterase (PNAE) catalyses a central reaction transforming into epi-vellosimine, which immediate precursor for synthesis ajmalane skeleton. PNAE was purified from cell suspension cultures Rauvolfia serpentina. N-terminal sequence and endoproteinase LysC fragments protein were used primer design amplification PCR products leading to isolation PNAE-encoding cDNA R. serpentina library. fused with C-terminal His-tag, expressed in Escherichia coli homogeneity using Ni-affinity chromatography. pure enzyme shows extraordinary substrate specificity, completely different other esterases. Sequence alignments indicate that new member alpha/beta hydrolase super family.
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