Lesion-induced gelsolin upregulation in the hippocampus following entorhinal deafferentation

摘要: Gelsolin is an actin-binding protein that regulates actin filament-severing and capping activity in the various processes of cell motilities. Here, we report expression gelsolin mRNA hippocampus following transections entorhinal afferents. Northern blot analysis showed transcript was upregulated a transient manner deafferented by 1.3-, 2.1-, 1.7-, 1.1- folds controls, respectively, at 1, 3, 7, 15 days postlesion (dpl). In situ hybridization immunohistochemistry confirmed temporal specifically entorhinally denervated zones: stratum lacunosum-molecular (SLM) outer molecular layer (OML) dentate gyrus (DG), which initiated as early 1 dpl, reached maximum 3 remained prominently elevated 7 discernibly higher dpl than controls. Double labeling either or with markers glial cells (Griffonia simplicifolia IB4 CD11b for microglial cells, GFAP astroglial cells) revealed highly expressed both activated microglia astrocytes. The results suggest spatiotemporal upregulation induced deafferentation, would participate activation thereby, indirectly play important roles subsequent lesion-induced neural reorganization deafferentation. © 2005 Wiley-Liss, Inc.