Interaction of Hepatitis C Virus Core Protein with Viral Sense RNA and Suppression of Its Translation

摘要: To clarify the binding properties of hepatitis C virus (HCV) core protein and its viral RNA for encapsidation, morphogenesis, replication HCV, specific interaction HCV with genomic synthesized in vitro was examined an vivo system. The positive-sense from 5′ end to nucleotide (nt) 2327, which covers untranslated region (5′UTR) a part coding structural proteins, interacted protein, while no observed same negative-sense other regions antiviral sense RNAs. internal ribosome entry site (IRES) exists around 5′UTR HCV; therefore, this suggests that there is some effect on cap-independent translation. Cells expressing were transfected reporter RNAs consisting nt 1 709 (the about two-thirds regions) followed by firefly luciferase gene (HCV07Luc RNA). translation HCV07Luc suppressed cells whereas significant suppression case possessing IRES encephalomyocarditis luciferase. This occurred dose-dependent manner. expression E1 envelope or β-galactosidase did not suppress both EMCV We then are important found 344 enough exert suppression. These results suggest interacts at form nucleocapsids regulates interacting 5′UTR.