CCL4 as an adjuvant for DNA vaccination in a Her2/neu mouse tumor model.

摘要: Chemokines are key regulators of both innate and adaptive immune responses. CCL4 (macrophage inflammatory protein-1β, MIP-1β) is a CC chemokine that has broad spectrum target cells including immature dendritic cells, which express the cognate receptor CCR5. We asked whether plasmid encoding able to improve tumor protection responses in Her2/neu+ mouse model. Balb/c mice were immunized twice intramuscularly with DNA on days 1 15. On day 25, challenge was performed 2 × 10(5) syngeneic D2F2/E2 cells. Different groups vaccinated pDNA(Her2/neu) plus pDNA(CCL4), pDNA(Her2/neu), pDNA(CCL4) or mock vector alone. Our results show (i) (ii) augment TH1-polarized response against Her2/neu. Although Her2/neu-specific humoral T-cell comparable induced previous studies using CCL19 CCL21 as adjuvants, conferred by inferior. Whether this due different (innate) remains be clarified. However, combination CCL19/21 might reasonable approach future, particularly for vaccination breast cancer situation minimal residual disease.