Pathogenesis of hepatitis A in orally inoculated owl monkeys (Aotus trivirgatus)

摘要: The pathogenesis of hepatitis A virus (HAV) infection was studied in owl monkeys following oral administration the wild-type HM-175 strain HAV. Stools were collected daily and blood pharyngeal swabs twice weekly for viral isolation, animals necropsied at various intervals after inoculation. Organs examined presence by isolation cell culture antigens immunofluorescence. Monkeys excreted HAV stools 1-4 days inoculation, presumably due to residual unabsorbed inoculum. No found next 2-3 days. re-appeared on 4-7 then persisted through day 39. Viremia occurred 10th continued until 35. Virus isolated occasionally from throat 1 or 2 weeks it detected blood, there no evidence that replicated tissues. Animals acquired anti-HAV antibody 4th week, alanine aminotransferase (ALT) elevated 5-5.5 liver 5 inoculation; however, first Kupffer cells 14 hepatocytes 21 antigen epithelial intestinal crypts lamina propria small intestine 3 postinoculation thereafter 5th suggesting these might represent an additional site replication.