Defining the morphological phenotype: 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) is a novel marker for in situ detection of canine but not rat olfactory ensheathing cells

摘要: Olfactory ensheathing cells (OECs) are the non-myelinating glial of olfactory nerves and bulb. The fragmentary characterization OECs in situ during normal development may be due to their small size requiring intricate ultrastructural analysis fact that available markers for detection either expressed only by OEC subpopulations or lost development. In present study, we searched with stable expression investigated spatiotemporal distribution CNPase, an early oligodendrocyte/Schwann cell marker, comparison prototype marker p75NTR. Anti-CNPase antibodies labeled canine but not rat situ, while Schwann oligodendrocytes were positive both species. CNPase immunoreactivity dog was confined all throughout postnatal associated entire body, including its finest processes, p75NTR mainly detected perineural some neonatal OECs. Adult bulb slices displayed after 4 10 days, detectable days vitro. Finally, treatment purified adult fibroblast growth factor-2 significantly reduced at protein mRNA level. Taken together, conclude is a suitable visualization will facilitate light-microscopic challenge our general view situ. supports idea glia from large animals differs substantially rodents.