Whole slide images for primary diagnostics of paediatric pathology specimens: A feasibility study

摘要: Introduction Whole slide images (WSI) have been used in many pathology applications such as teleconsultation, teaching and research, but not primary diagnostics. The aim of this study was to test the feasibility using WSI diagnostics paediatric specimens placental tissue. Materials methods Eighty consecutive tissues biopsies resections from patients under 18 years old were selected, well 20 placentas. These cases had diagnosed year 2009 by a single pathologist. same pathologist who performed original diagnosis based on light microscopy asked rediagnose these 100 scanned at 20× magnification having clinical information available, blinded microscopic diagnoses. diagnoses compared with rediagnoses classified concordant, mildly discordant (without consequences) (with consequences). Results concordant 90% 93% respectively, which significantly different. Digital reassessment yielded eight mild discrepancies two discrepant (2%) where difference could implications for patient. Light showed seven discrepancies. It turned out be difficult identify nucleated red blood cells WSI, even when 40×. Conclusions Primary tissue can generally done WSI. However, some difficulties encountered examining placenta identification may need higher resolution or scanning multiple focus depths, is possible most current scanners.