Usage of the Heterologous Expression of the Antimicrobial Gene afp From Aspergillus giganteus for Increasing Fungal Resistance in Olive.
作者： Isabel Narvaez , Titouh Khayreddine , Clara Pliego , Sergio Cerezo , Rafael M. Jiménez-Díaz
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摘要: The antifungal protein (AFP) produced by Aspergillus giganteus, encoded the afp gene, has been used to confer resistance against a broad range of fungal pathogens in several crops. In this research, transgenic olive plants expressing gene under control constitutive promoter CaMV35S were generated and their disease response two root infecting pathogens, Verticillium dahliae Rosellinia necatrix, was evaluated. Embryogenic cultures derived from mature zygotic embryo cv. 'Picual' for A. tumefaciens transformation. Five independent lines obtained, showing variable level expression leaves roots. None these showed enhanced wilt. However, some displayed degree incomplete white rot caused R. necatrix compared with reaction non-transformed or only GUS gene. pathogen correlated that leaves. Our results indicate can be useful partial olive, but does not protect V. dahliae.
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José A. Mercado, Marta Barceló, Clara Pliego, Manuel Rey, José L. Caballero, Juan Muñoz-Blanco, David Ruano-Rosa, Carlos López-Herrera, Berta de los Santos, Fernando Romero-Muñoz, Fernando Pliego-Alfaro, Expression of the β-1,3-glucanase gene bgn13.1 from Trichoderma harzianum in strawberry increases tolerance to crown rot diseases but interferes with plant growth. Transgenic Research. ,vol. 24, pp. 979- 989 ,(2015) , 10.1007/S11248-015-9895-3
Jyothi Prakash Bolar, John L. Norelli, Gary E. Harman, Susan K. Brown, Herb S. Aldwinckle, Synergistic activity of endochitinase and exochitinase from Trichoderma atroviride (T. harzianum) against the pathogenic fungus (Venturia inaequalis) in transgenic apple plants Transgenic Research. ,vol. 10, pp. 533- 543 ,(2001) , 10.1023/A:1013036732691
P.A. Roussos, C.A. Pontikis, In vitro propagation of olive (Olea europaea L.) cv. Koroneiki Plant Growth Regulation. ,vol. 37, pp. 295- 304 ,(2002) , 10.1023/A:1020824330589
CLARA PLIEGO, CARLOS LÓPEZ-HERRERA, CAYO RAMOS, FRANCISCO M. CAZORLA, Developing tools to unravel the biological secrets of Rosellinia necatrix, an emergent threat to woody crops Molecular Plant Pathology. ,vol. 13, pp. 226- 239 ,(2012) , 10.1111/J.1364-3703.2011.00753.X
F.J. López-Escudero, C. del Río, J.M. Caballero, M.A. Blanco-López, Evaluation of olive cultivars for resistance to Verticillium dahliae European Journal of Plant Pathology. ,vol. 110, pp. 79- 85 ,(2004) , 10.1023/B:EJPP.0000010150.08098.2D
Maria C. Gomez-Jimenez, Miguel A. Paredes, Mercedes Gallardo, Nieves Fernandez-Garcia, Enrique Olmos, Isabel M. Sanchez-Calle, Tissue-specific expression of olive S-adenosyl methionine decarboxylase and spermidine synthase genes and polyamine metabolism during flower opening and early fruit development Planta. ,vol. 232, pp. 629- 647 ,(2010) , 10.1007/S00425-010-1198-6
Sergio Cerezo, José A. Mercado, Fernando Pliego-Alfaro, An efficient regeneration system via somatic embryogenesis in olive Plant Cell Tissue and Organ Culture. ,vol. 106, pp. 337- 344 ,(2011) , 10.1007/S11240-011-9926-6
Rocío Torreblanca, Sergio Cerezo, Elena Palomo-Ríos, José A. Mercado, Fernando Pliego-Alfaro, Development of a high throughput system for genetic transformation of olive ( Olea europaea L.) plants Plant Cell Tissue and Organ Culture. ,vol. 103, pp. 61- 69 ,(2010) , 10.1007/S11240-010-9754-0
Ramon Campos-Olivas, Marta Bruix, Jorge Santoro, Javier Lacadena, Alvaro Martinez del Pozo, Jose G. Gavilanes, Manuel Rico, NMR solution structure of the antifungal protein from Aspergillus giganteus: evidence for cysteine pairing isomerism Biochemistry. ,vol. 34, pp. 3009- 3021 ,(1995) , 10.1021/BI00009A032
Gema Pérez-Barranco, Rocío Torreblanca, Isabel M. G. Padilla, Carolina Sánchez-Romero, Fernando Pliego-Alfaro, José A. Mercado, Studies on genetic transformation of olive (Olea europaea L.) somatic embryos: I. Evaluation of different aminoglycoside antibiotics for nptII selection; II. Transient transformation via particle bombardment Plant Cell Tissue and Organ Culture. ,vol. 97, pp. 243- 251 ,(2009) , 10.1007/S11240-009-9520-3