Limited dispersion and quick degradation of environmental DNA in fish ponds inferred by metabarcoding

摘要: Environmental DNA (eDNA) metabarcoding is a promising tool for rapid, non-invasive biodiversity monitoring. In this study, eDNA applied to explore the spatial and temporal distribution of in two ponds following introduction removal rare fish species. When species were introduced kept at fixed location ponds, concentration (i.e., proportional read counts abundance) typically peaked after days. Thereafter, it gradually declined stabilised six These findings are supported by highest community dissimilarity different sampling positions being observed on second day introduction, which then decreased over time. On sixth day, there was no longer significant difference between The detected any 48 hrs from ponds. signal detection probability strongest near keepnets, resulting variance events position. significantly with increasing distance, although increased slightly again 85 m position away keepnets. Collectively, these reveal that lentic ecosystems highly localised space time, adds growing weight evidence provides good approximation presence Moreover, powerful alongside more abundant due use generic PCR primers, can enable monitoring variance.