ANALYSIS OF THE CONCENTRATED SOLUTION PROPERTIES OF HYALURONAN BY CONFOCAL-FRAP SHOW NO EVIDENCE OF CHAIN-CHAIN ASSOCIATION

摘要: The network and hydrodynamic properties of fluoresceinamine labelled hyaluronan (FA-HA) was investigated at up to 10 mg/ml by confocal fluorescence recovery after photobleaching (confocal-FRAP). influence electrolytes on self tracer lateral diffusion coefficients showed that in Ca2+ Mn2+, FA-HA (830 kDa) more compact than Mg2+, Na+ or K+. These results correlated with changes the radius HA, determined multi-angle laser light scattering analysis (MALLS) dilute solution, which smaller CaCl2 (36 nm) NaCl (43 nm). permeability HA solutions (<10 mg/ml) FITC-dextran tracers (2000 also higher a reduced size increased urea (up 6M) ethanol/water 62 % vol/vol). Both solvents did not show predicted if chain-chain association disrupted urea, enhanced ethanol. Oligosaccharides (HA20–26) had no effect high molecular weight solutions, dextran diffusion, showing there were interactions open competition short chain segments. suggest physical are primarily solvent effects flexibility, entanglement dominating concentrated evidence for between segments making significant contribution.