Advances in venomics: Modern separation techniques and mass spectrometry.

摘要: Snake venoms are complex chemical mixtures of biologically active proteins and non-protein components. Toxins have a wide range targets effects to include ion channels membrane receptors, platelet aggregation plug formation. target these effectors at high affinity selectivity. From pharmacological perspective, snake venom compounds valuable resource for drug discovery development. However, major challenge using is isolating analyzing the bioactive peptides in mixtures. Getting molecular information from such as requires proteomic analyses, generally combined with transcriptomic analyses glands. The present review summarizes current knowledge highlights important recent advances venomics special emphasis on contemporary separation techniques bioinformatics that begun elaborate complexity venoms. Several analytical two-dimensional gel electrophoresis, RP-HPLC, size exclusion chromatography, exchange MALDI-TOF-MS, LC-ESI-QTOF-MS been employed this regard. improvement approaches multidimensional-HPLC, 2D-electrophoresis coupled soft-ionization (MALDI ESI) mass spectrometry has critical obtain an accurate picture startling In case bioinformatics, variety software tools PEAKS also used successfully. Such gleaned both predicting resolving biological activity components venoms, which turn key development new drugs based