A method for paraffin sectioning and identification of indoleamines in the brain of insects with a sclerotized cuticle

摘要: AbstractSectioning of insects with sclerotized cuticles for histological examination can be challenging and often involves double-embedding, chemical dissolution the cuticle or long periods sample preparation. Furthermore, exposure tissue to harsh treatments potentially interferes antibody binding thus is undesirable performing fluorescence immunohistochemistry (IHC). A method obtaining paraffin sections compound eye neural from Australian black field cricket Teleogryllus commodus, an Orthopteran insect a cuticle, without need double embedding minimal pre-embedding processing presented here. This produced curling lifting in tissues interest. Fluorescence IHC labelling melatonin serotonin, comprehensively demonstrating their distribution optic lobe brain, was also performed. ...