Characterization of human fetal cord blood steroid profiles in relation to fetal sex and mode of delivery using temperature-dependent inclusion chromatography and principal component analysis (PCA)

摘要: In the present work, human male and female fetal cord blood samples were purified, selectively extracted separated to examine a fraction of steroids ranging from polar estetrol relatively non-polar progesterone using solid phase extraction based on C-18 tubes β-cyclodextrin driven temperature dependent inclusion chromatography. Resulting UV diode array chromatographic patterns revealed presence 27 peaks. Chromatographic detected analyzed principal components analysis which differences between male/female labour/not-in-labour clusters. Quantitative nine identified including: estetrol, 17β-estradiol, estrone, estriol, cortisol, cortisone, progesterone, 20α-hydroxyprogesterone 17α-hydroxyprogesterone not significantly different males females. Significant fetuses related as yet unidentified compounds. Four peaks with labour corresponded cortisone two This protocol may distinguish significant clinical groups that are readily identifiable univariate measurements single or low molecular mass biomarkers. Moreover, we have provided new evidence despite absence testosterone there number compounds differ fetuses.