Transforming growth factor-betas and CD105 expression in calcification and bone formation in human atherosclerotic lesions.

摘要: Objectives. To investigate the expression and localisation of transforming growth factor betas (TGFβs) their receptor CD105 (endoglin) in relation to calcification bone formation atherosclerotic lesions human carotid arteries. Background. The TGFβ family regulates cellular growth, differentiation angiogenesis plays a key role enchondral formation. is part complex preferentially expressed on endothelial cells (EC). Methods. Immunohistochemical methods were used determine isoforms 1, 2, 3 spatial patterns lesions. Cellular sources TGFβs assessed using cell-type specific antibodies. Results. There was marked variability different cell types associated with calcification. Smooth muscle (SMC) atheroma cap showed higher levels TGFβ3 2 than but deep musculoelastic intima there TGFβ1 α-actin. All three monocyte-macrophages. Giant calcifications intense staining for TGFβ2. most strongly matric SMCs monocyte-macrophages lower close association high CD105. Conclusions. exhibit distinct overlapping expression, support hypothesis that they are involved process Lower may represent state responsiveness TGFβs.