Poliovirus 2b insertion into lipid monolayers and pore formation in vesicles modulated by anionic phospholipids.

摘要: Enterovirus 2B viroporin has been involved in membrane permeabilization processes occurring late during cell infection. Even though lacks an obvious signal sequence for translocation, the presence of a Lys-based amphipathic domain suggests that this product bears intrinsic capacity partitioning into negatively charged cytofacial surfaces. Pore formation by poliovirus attached to maltose-binding protein (MBP) indeed demonstrated pure lipid vesicles, fact supporting spontaneous insertion and direct membranes. Here, biochemical evidence is presented indicating both are modulated phosphatidylinositol phosphatidylserine, main anionic phospholipids existing membranes target organelles. Insertion monolayers phospholipid bilayers were sustained phospholipids. However, MBP-2B inserted phosphatidylserine did not promote addition inhibited leakage observed vesicles. Mathematical modelling pore containing increasing percentages was consistent with its inhibitory effect arising from higher reversibility surface aggregation. These results support pore-opening mechanistically distinguishable events