Drosophila polypyrimidine‐tract binding protein (PTB) functions specifically in the male germline

摘要: The mammalian polypyrimidine-tract binding protein (PTB), which is a heterogeneous ribonucleoprotein, ubiquitously expressed. Unexpectedly, we found that, in Drosophila melanogaster, the abundant PTB transcript present only males (third instar larval, pupal and adult stages) flies restricted to germline. Most importantly, signal from somatic sex-determination pathway that dependent on male-specific isoform of doublesex (DSXM) regulates PTB, providing evidence for necessity soma–germline communication differentiation male Analysis P-element insertion directly links function with fertility. Specifically, loss dmPTB affects spermatid differentiation, resulting accumulation cysts elongated spermatids without producing fully separated motile sperms. This expression conserved D.virilis. Thus, appears be particularly potent downstream target germline, since it can regulate multiple mRNAs.