Silver nanoparticles promote osteogenic differentiation of human periodontal ligament fibroblasts by regulating the RhoA-TAZ axis.
作者： Yunhe Xu , Bowen Zheng , Jia He , Zhao Cui , Yi Liu
DOI: 10.1002/CBIN.11180
关键词:
摘要: Silver nanoparticles (AgNps) are well established antibacterial agents, which have been reported to promote osteogenesis of stem cells. Ras homolog gene family member A (RhoA) and Tafazzin (TAZ) recently shown be involved in osteogenic differentiation. The present study aimed evaluate the effects AgNps on differentiation human periodontal ligament fibroblasts (HPDLFs), investigate underlying mechanisms activity. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was employed determine safe concentration HPDLFs. Using an alkaline phosphatase assay, Alizarin red S staining detection expression (ALP), runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), osterix (OSX), collagen-I, we found that AgNps, at a range 25-100 μM, promoted HPDLFs dose-dependent manner. We also 100 μM up-regulated active RhoA level. results ALP activity markers showed were abrogated by pathway inhibitor, C3 reagent. Additionally, silencing TAZ attenuated AgNps-induced HPDLFs, as decreased down-regulation markers. Interestingly, knockdown had very small effect RhoA, whereas suppressed TAZ, indicating downstream effector RhoA. In conclusion, work demonstrated activating RhoA-TAZ axis.
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