Solvent free biocatalytic synthesis of isoniazid from isonicotinamide using whole cell of Bacillus smithii strain IITR6b2

摘要: Abstract A biocatalytic route for the synthesis of isoniazid, an important first-line antitubercular drug, in aqueous system is presented. The reported bioprocess a greener method, does not involve any hazardous reagent and takes place under mild reaction conditions. Whole cell amidase Bacillus smithii strain IITR6b2 having acyltransferase activity was utilized its ability to transfer acyl group isonicotinamide hydrazine–2HCl medium. B. possessed 3 folds higher as compared amide hydrolase this ratio further improved 4.5 by optimizing concentration co-substrate hydrazine–2HCl. Various key parameters were optimized optimum conditions pH (7, phosphate buffer 100 mM), temperature (30 °C), substrate/co-substrate (100/1000 mM) resting cells (2.0 mg dcw /ml), 90.4% conversion isoniazid achieved 60 min. Under these conditions, fed batch process production developed resulted accumulation 439 mM with 87.8% molar yield productivity 6.0 g/h/g . These results demonstrated that enzymatic using whole might present efficient alternative chemical procedures without involvement organic solvent.