Anhydrobiotic potential and long-term storage of entomopathogenic nematodes (Rhabditida: Steinernematidae).

摘要: Abstract Anhydrobiosis is considered to be an important means of achieving storage stability entomopathogenic nematodes that are used in biological control. This study explored the effects anhydrobiosis on longevity and infectivity infective juveniles (IJs) three species Steinernema carpocapsae , feltiae riobrave at 5 25°C. was induced water-dispersible granules (WG) 0.966–0.971 water activity 25°C following a 7-day preconditioning IJs 5°C tap water. Survival desiccated (anhydrobiotic) compared with non-desiccated stored for different periods. increased S. by 3 months 1 month WG as However, desiccation decreased all 5°C. These results demonstrate shelf-life 9 over 90% IJ survival. For feltiae, survival occurred only 2 WG. had dropped below 85% within Induction resulted 85, 79 76% reduction oxygen consumption IJs, respectively. Differences among were related both initial lipid content rate utilisation, but not The one-on-one infection bioassays indicated no negative effect any nematode suggesting harmful and/or their symbiotic bacteria. differences temperatures discussed relation foraging strategy temperature adaptation.