Gene expression profiling supports the hypothesis that human ovarian surface epithelia are multipotent and capable of serving as ovarian cancer initiating cells

摘要: Accumulating evidence suggests that somatic stem cells undergo mutagenic transformation into cancer initiating cells. The serous subtype of ovarian adenocarcinoma in humans has been hypothesized to arise from at least two possible classes progenitor cells: the surface epithelia (OSE) and/or an as yet undefined class residing distal end fallopian tube. Comparative gene expression profiling analyses were carried out on OSE removed normal human ovaries and epithelial (CEPI) isolated by laser capture micro-dissection (LCM) papillary adenocarcinomas. results randomly confirmed paraffin embedded tissues non-neoplastic using immunohistochemistry. Differentially expressed genes analyzed ontology, molecular pathway, set enrichment analysis algorithms. Consistent with multipotent capacity, pathways previously associated adult cell maintenance are highly not or very low levels adenocarcinoma. Among over 2000 significantly differentially expressed, a number novel pathway interactions identified may contribute development. Our consistent hypothesis capable serving origin While our findings do rule possibility cancers also other sources, they inconsistent claims cannot serve