Variation in H-2 antigen expression on lymphomyeloid cells in semi-allogeneic irradiation chimeras.

摘要: Spleen cells from 30 individual murine irradiation chimeras of the type (P1 X P2)F1----P1 were compared in a rosetting assay for H-2K and H-2D cell surface antigen expression with normal P2)F1 hybrid controls. Eleven out range, but other 19 differed F1 controls by 4- to 100-fold endpoint titre at least one or antigen. Every possible class variation was found, i.e. up down antigens P1 P2 type. This evidence, together data T6 chromosome marker experiments which also showed full reconstitution lethally irradiated recipients donor lymphomyeloid stem cells, suggested that incomplete not cause H-2 antigenic variation. Low on spleen derived investigated further. Fifteen bone marrow two such all phenotype when tested 10-12 weeks after reconstitution, thus excluding stable, low H-2-expressing variant as phenomenon. If hyperimmunized against before lethal there significantly fewer Till- McCulloch colonies their spleens 10 days than unimmunized Also greater 90% immunized died 6 injection survivors both very levels antigens. These results previously published evidence anti-P2 Tc activity skin graft rejection residual immunological capability may be associated F1-derived although mechanism does involve selection cells. The mechanism(s) classes investigated.